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Fig. 6 | Virology Journal

Fig. 6

From: Emerging zoonotic potential of H4N1 avian influenza virus: enhanced human receptor binding and replication via novel mutations

Fig. 6

Replication kinetics of H4N1 AIVs in A549 (a) and DF-1 (b) cells. A549, DF-1 were inoculated in triplicate with an MOI of 0.01. Post-inoculation, the cells were incubated at 37 °C, and culture supernatants were collected at 12, 24, 36, 48, 60, and 72 hpi for viral titer measurement. Viral titers were determined by titration in MDCK cells and expressed as TCID50. Each data point represents the mean of three independent replicate experiments. Statistical significance between viral load at each hpi was analyzed by two-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test (Supplementary Table S2)

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Virology Journal

ISSN: 1743-422X