Fig. 4
From: HPV16 E7 inhibits HBD2 expression by down-regulation of ASK1-p38 MAPK pathway in cervical cancer
HPV16 E7 down-regulated the ASK1-p38 MAPK signaling pathway in cervical cancer cells. 50 nM HPV16 E7 siRNA sequence or random control RNA sequence were tranfected into SiHa (A) and CaSki cells (B) for 36 h, while 3 µg pcDNA3.1(-)-3FLAG-HPV16 E7 plamids or empty control plasmids were transfected into C33A (C) and CaCo2 cells (D) for 48 h, the protein levels of HPV16 E7, ASK1, p-ASK1(Thr845), p-ASK1(Ser966), p38 and p-p38 in all cells were measured by Western blot analysis. The cells in control groups were not treated with any RNA sequence and plasmid. The relative expression of all proteins was quantified using Image J software, with GAPDH as an internal control. *P < 0.05, **P < 0.01