Table 1 Nucleotide sequences of the primer pairs used in the quantitative real-time PCR assays performed in this study
Target gene | Forward primer | Reverse primer |
|---|---|---|
IFNβ Interferon beta | gcagtctgcacctgaaaagatatt | tgtactccttggccttgaggta |
IFNλ1 (IL29) Interferon Lambda 1 | ggaagagtcactcaagctgaaaaac | agaagcctcaggtcccaattc |
IFNλ2/3 (IL-28 A/B) Interferon Lambda 2/3 | acatagcccagttcaagtc | gactcttctaaggcatctttg |
RPLP0 Ribosomal Protein Lateral Stalk Subunit P0 | agatgcagcagatccgcat | ggatggccttgcgca |
IFI6 Interferon Alpha Inducible Protein 6 | ctgaagattgcttctcttctc | cactttttcttacctgcctc |
IFIT1 Interferon Induced Protein With Tetratricopeptide Repeats 1 | aacttaatgcaggaagaacatgacaa | ctgccagtctgcccatgtg |
IFIT2 Interferon Induced Protein With Tetratricopeptide Repeats 2 | agaaagctgatgaggccaatg | gcatggaggctggcaaga |
IFITM1 Interferon Induced Transmembrane Protein 1 | cgccaagtgcctgaacatct | cccgtttttcctgtattatctgta |
ISG15 interferon stimulated gene 15 ubiquitin like modifier | tggcgggcaacgaatt | gggtgatctgcgccttca |
MX1 Myxoma resistance Dynamin Like GTPase 1 | cagcacctgatggcctatcac | catgaagaactggatgatcaaagg |
RSAD2 (Viperin) Radical S-Adenosyl Methionine Domain Containing 2 | gtgagcaatggaagcctgatc | gctgtcacaggagatagcgagaa |
Zika Virus (PRVABC59) | gtgtgatgccaccatgagcta | tggcaggttccgtacacaac |
Dengue Virus-2 (16681) | caatatgctgaaacgcgagaga | accagggccatgaacagtttta |