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Table 2 Primers used for UL32 FHV-1 RT-qPCR

From: Transcriptomic profiles of Crandell-Rees feline kidney cells infected with Varicellovirus felidalpha-1 (FHV-1) field and vaccine strains

Primer

Direction

Sequence (5’ → 3’)

Binding site (nt)1

UL32

Forward

CCATACCACTCTGTGCCACC

46,212–46,231

Reverse

GAACGCCCCCACCAAAGTAA

46,318–46,299

UL33

Forward

AACGTGATTTTTGCGTGGCC

45,525–45,544

Reverse

TTATCATATACCCAGCGACTCG

45,624–45,603

  1. 1 Nucleotide numbers of the binding site relate to the alignment of the FHV-1 F2 vaccine strain (Feligen) (Genbank Accession No. KR296657)
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Virology Journal

ISSN: 1743-422X