Table 1 Characteristics of antibodies used for immunofluorescence stainings

Ciliated cells

Acetylated-α-tubulin (AF647), clone 6-11B-1, Cat. No. SC-23950 (Santa Cruz)

Mouse IgG2β

1:100 (2 µg/mL)

1 h at RT

Goblet cells

Gastric muc5AC, clone 45M1, Cat. No. M5293 (Sigma Aldrich)

Mouse IgG1

1:780 (10 µg/mL)

Overnight at 4 °C

Tight junctions

ZO-1 (AF555), clone 1A12, Cat. No. MA3-39,100 (ThermoFisher Scientific)

Mouse IgG1

1:100 (10 µg/mL)

1 h at RT

Tight junctions

Occludin, Cat. No. 71–1500 (ThermoFisher Scientific)

Rabbit polyclonal

1:100 (2.5 µg/mL)

Overnight at 4 °C

Tight junctions

Claudin 5, Cat. No 34–1600 (ThermoFisher Scientific)

Rabbit polyclonal

1:100 (2.5 µg/mL)

Overnight at 4 °C

Basal cells

P63, clone 4A4, Cat. No. ab735 (Abcam)

Mouse IgG2α

1:50 (0.5 µg/mL)

Overnight at 4 °C

Mouse IgG

Goat anti-Mouse IgG (H + L) Cross-Adsorbed (AF488), Cat. No. A-11001 (ThermoFisher Scientific)

Goat polyclonal

1:400 (5 µg/mL)

1 h at RT

Rabbit IgG

Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed (AF488), Cat. No. A11034 (ThermoFisher Scientific)

Goat polyclonal

1:400 (5 µg/mL)

1 h at RT