Fig. 3

Detection of SPIKE protein, VEGF and FN by MICSSS. The sections of control ileum (A), non-ulcerated (B) and ulcerated ileum (C) were sequentially stained and scanned for H-E, SPIKE, VEGF, FN and the nuclei. Bright-field images were inverted, and RGB channel splitting was performed. The most significant images were merged, and pseudocolors were attributed to each marker. Scale bars, 200Â ÎĽm. The arrows indicate points of co-expression of the three markers analyzed in the tissue. MICSSS, Multiplexed Immunohistochemical Consecutive Staining on Single Slide