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Table 1 The qPCR and nested RT-PCR primers/probe sequences of BRSV that were used in this study

From: A surrogate in vitro experimental model for off-label drug repurposing: inhibitory effect of montelukast on bovine respiratory syncytial virus replication

Primers or probe

Sequence 5′–3′

The product size(bp)

References

F primer*

GCAATGCTGCAGGACTAGGTATAAT

124

[14]

R primer*

ACACTGTAATTGATGACCCCATTCT

  

Probe*

FAM-ACCAAGACTTGTATGATGCTGCCAAAGCA-TAMRA

  

B5A**

CCA CCC TAG CAA TGA TAA CCT TGA C

603

[15]

B6A**

AAG AGA GGA TGC (T/C)TT GCT GTG G

  

B7A**

CAT CAA TCC AAA GCA CCA CAC TGT

371

 

B8**

GCT AGT TCT GTG GTG GAT TGT TGT C

  
  1. *Real-time RT-PCR primers and probe for the N gene of BRSV
  2. **Nested -PCR primers for the G gene of BRSV
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Virology Journal

ISSN: 1743-422X