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Fig. 2 | Virology Journal

Fig. 2

From: The immunogenicity of PRV ΔgE/TK/UL49.5 three-gene-deleted vaccine in mice

Fig. 2

Purification and identification of PRV strains. (A) Plaque purification of PRV ΔgE/TK/UL49.5/EGFP+ strain for fluorescence visual field. (B) Plaque purification of PRV ΔgE/TK/UL49.5/EGFP+ strain for bright visual field, red circles represent the plaque of PRV ΔgE/TK/UL49.5/EGFP+. (C) Plaque purification of PRV ΔgE/TK/UL49.5 strain for fluorescence visual field. (D) Plaque purification of PRV ΔgE/TK/UL49.5 strain for bright visual field, red circles represent the plaque of PRV ΔgE/TK/UL49.5. (E) PCR analysis of different PRV strains with primers UL49.5-F/R. Lane 1, PRV ΔgE/TK/UL49.5; lane 2, PRV ΔgE/TK/UL49.5/EGFP+; lane 3, PRV QYY2012; lane 4, negative control; M, DL2000 DNA Marker

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Virology Journal

ISSN: 1743-422X