Fig. 4

Effect of URI1 overexpression on HBV infection in HepG2-NTCP cells. (A) Experimental timeline. (B) URI1 mRNA expression following overexpression was determined by RT-qPCR 6 days after infection. Data represent the mean ± SD; triangles indicate the means of independent experiments. Data are shown on a logarithmic scale. (C) URI1 and HA-URI1 protein levels were analyzed by western blotting 1 and 6 days after infection. HBc protein level was detected 6 days after infection. Equal protein loading was confirmed by detecting GAPDH or vinculin on the same membrane. Saturated pixels are highlighted in red. (D-E) Viral antigen secretion and RNA production were evaluated 6 days after infection. Data represent the mean ± SD of four independent experiments; replicates from independent experiments are differentiated by color, with triangles indicating the means of independent experiments. (D) HBsAg and HBeAg levels in the culture medium were measured by CLIA assay. (E) Total HBV RNA and HBV 3.5 kb RNA expression were determined by RT-qPCR