Fig. 2

Antiviral activity of Ae against ASFV (A and C) The ASFV B646L gene copy-number in the ASFV-infected group and Ae-treated ASFV-infected group was detected at different time points by qPCR. The results of three independent experiments (mean ± SD) were represented by one data. (B and D) The ASFV p30 protein expression level in the ASFV-infected group or Ae-treated ASFV-infected group were detected by western blotting. (E) IFA was used to detect the expression level of p30 protein in the control group, the ASFV-infected group and the Ae-treated ASFV-infected group, and the antiviral activity of Ae against ASFV was determined. The results of three independent experiments (mean ± SD) were represented by one data. The graph above is representative of three independent IFA trials with similar results. Significant differences that were compared to the control group were indicated by * (P < 0.05), ** (P < 0.01) and *** (P < 0.001). ASFV, African swine fever virus; qPCR, real-time polymerase chain reaction; IFA, Indirect Immunofluorescence Assay